United Nation officials say, Hundreds of men appear to have gone missing after crossing from rebel-held areas of Aleppo into government territory.
Forces led by Syria's government have seized at least 75% of eastern parts of the city from rebels in recent weeks.
Tens of thousands of civilians have fled those districts, with more than 8,000 leaving during a humanitarian pause on Thursday.
Rebels were also reportedly stopping people from leaving, the UN said.
What are the allegations?
Rupert Colville, the spokesman for the UN High Commissioner for Human Rights, said up to 100,000 people were trapped in "ever-shrinking" areas of eastern Aleppo.
Reports differ on how many people remain and how many have fled eastern Aleppo, but Mr Colville said the UN had gathered evidence that "hundreds" of men may have disappeared after leaving for government-held areas.
"Given the terrible record of arbitrary detention, torture and enforced disappearances by the Syrian government, we are of course deeply concerned about the fate of these individuals," he said.
Reports cited by the UN say men aged between 30 and 50 were separated from their families. Other displaced people reported being taken in for questioning and having their identity cards confiscated.
Mr Colville also said rebel groups could be committing a war crime by preventing people from fleeing to safety, and "using civilians as pawns".
What's happening on the ground?
On Thursday, Russia, an ally of Syrian President Bashar al-Assad, said fighting was suspended to allow civilians to leave.
By Friday morning, more than 8,000 people - including 3,000 children - had left, Russia's military said. But fighting would continue, Russian Foreign Minister Sergei Lavrov said, "until the bandits leave east Aleppo".
The UK-based Syrian Observatory for Human Rights, a network of activists monitoring the violence, said ground forces continued their offensive, and that rocket attacks and air raids were launched overnight.
Meanwhile, the civilian rescue group known as the White Helmets said 46 civilians were killed and another 230 injured on Thursday in eastern Aleppo. Three barrel bombs carrying chlorine gas were dropped, it added.
After several previous attempts to flee the besieged area of Salhine, one man, Abdel Hamid, managed to leave with his wife and ten children on Thursday.
"Most of the people around me were saying 'in any case we will die, so let's leave together'. That encouraged me, and we left," he told the AFP news agency.
"I left my house behind... but I have secured my children's right to live. With each step I took, I felt like I was getting closer to life itself."
Mr Lavrov confirmed that Russian and US military experts would meet in Geneva on Saturday to discuss ways of ending the violence.
He said the talks would focus on plans to evacuate rebel fighters from eastern Aleppo, but the US state department said the subject had yet to be agreed. Rebels have said they intend to fight on.
The UN's envoy to Syria, Staffan de Mistura, told reporters in New York on Thursday that he was planning to meet members of US President-elect Donald Trump's team, though he did not say when.
Aleppo was once Syria's largest city and its commercial and industrial hub before the uprising against President Bashar al-Assad began in 2011.
It has been divided in roughly two since mid-2012. But in the past year, Syrian troops have broken the deadlock with the help of Iranian-backed militias and Russian air strikes, reinstating a siege in early September.
Correction: A previous version of this article mistakenly reported that the UN said boys, as well as men, were missing.
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